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Microbiology

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Mold Allergy: Reduce Exposure to Fungal Spores
Mold and mildew are fungi that produce tiny spores. Those who are allergic to mold can help manage symptoms by reducing exposure to spores both in the home and outdoors.
Mold Allergy: Symptoms, Testing and Treatment
Mold allergies are common and result in symptoms similar to other allergic reactions. Here is a summary of how allergies to mold and mildew are diagnosed and treated.
Mold Allergy: Which Fungi Cause Allergies?
Mold and mildew, like all fungi, produce tiny spores to reproduce. Fungal spores are found throughout the environment and can cause allergic reaction in some people.
Microscope Slide Preparation of Eukaryotic Cells
Preparing a wet mount of a specimen is the technique typically used to view plant and animal cells. Here is the step by step process of slide preparation.
Flies Live Longer on Low Protein Diets
A new study shows a strong correlation between longer life and low protein diets in Drosophila, or fruit flies. Low protein diets slow down mitochondrial degradation.
Basic Cytoskeletal Proteins
The stability of cell shape and structure are maintained by cytoskeletal proteins that interact with and transport required functional molecules.
How to Use a Compound Microscope
The following is a troubleshooting guide to the most common problems encountered when trying to view specimens with a compound microscope.
How to Properly Set Up a Compound Microscope
The following is a troubleshooting guide to the most common problems encountered when setting up a compound microscope to view specimens.
Difference between Plant and Animal Cells
Both plant and animal cells are eukaryotic, with many similarities, but there are also key differences between the cells of plants and animals. Here is a summary.
Symbiotic Relationships between Microbe and Host
There are three types of symbiotic relationships that microbes can have with their host organism: mutualism, commensalism and parasistism.
Domain Archaea, Bacteria-like Prokaryotes
Archaea are prokaryotes that differ from bacteria and eukaryotes enough to be assigned to their own taxonomic domain. Here is a brief introduction to Archaeans.
Difference between Aerobic & Anaerobic Bacteria
Oxygen is required for cells to break down organic molecules in the most energy-efficient way. How do microbes that live under conditions of low or no O2 metabolize food?
Sustainable Agriculture Using Biofertilizers
Environmental concerns have necessitated a fresh look at "clean and green" options in agriculture. Biofertilizers are the new "in thing."
Archaeans – Methanogens in Extreme Environments
Thought to be among the oldest organisms on earth, methanogens live in extreme environments and have adaptations allowing them to thrive in their harsh habitats.
Structural Differences of Bacteria and Viruses
Bacteria and viruses are both tiny infectious agents with the ability to cause disease. So it is not surprising that these microbes are sometimes mistaken as the same.
Expression of Genes in Bacteria and Viruses
Bacteria are living cells with DNA genomes that direct the production of enzymes required for metabolism. Viruses are not alive. What does the viral genome do?
How Viruses and Bacteria Infect Human Hosts
Both bacteria and viruses can cause disease. However, these two types of infectious agents achieve their pathogenicity in different ways.
Species Distinction and Prokaryotes
The distinction between species of sexually reproducing organisms is quite clear, but how are the lines drawn for organisms, like bacteria, that reproduce as clones?
How Are Streptococcal Species Classified?
Types of Streptococcus can be distinguished by cell arrangements, hemolysis pattern that they generate on blood agar and by their antigenic particles.
Difference between Group A and B Streptococcus
Bacteria in the genus Streptococcus can be classified by species, hemolysis pattern and by their antigens, chemicals to which the human immune system reacts.
What Causes Stomach Ulcers?
Think that stress and spicy food cause ulcers? Think again! Although these factors can make an ulcer worse, peptic ulcers are actually caused by the bacterium H. pylori.
Microbiology - Seeing & Understanding the Unseen
They are almost everywhere, in the air, soil and water. They are on and inside the bodies of living things. They can help, and they can harm. They are the microbes.
Fimbriae and Bacterial Virulence
Fimbriae are external structures of Gram- cells which enable bacteria to adhere to surfaces and, as virulence factors, mediate infection of host cells.
What Are Bacterial Fimbriae?
Most Gram-negative bacteria have hair-like projections external to their cell's wall. One type, fimbriae, allows bacteria to stick together and attach to host cells.
External Structures of Prokaryotic Cells
Prokaryotic cells can have a variety of surface appendages - flagellum, fimbria or pilus - that enable them to move, adhere to surfaces and even infect host cells.
What Is Croup Cough?
The term croup (pronounced kroop) refers to a hoarse barking cough resulting from inflammation of the upper airways; a condition typically caused by viral infection.
How to Interpret Beta Hemolysis on Blood Agar
What does it mean if a BAP throat culture shows B-hemolysis? Do you have strep throat? Maybe. Here is some additional information on B-hemolytic bacteria.
What are Normal Flora?
The human body is made up of about 10 trillion cells, but hosts 100 trillion more. The vast majority of cells living on and in the body are bacteria and other microbes.
Robert Koch – History of Microbiology
Frustrated by unfulfilled big dreams of high adventure, Robert Koch, a German physician, was destined for smallness, as a pioneer of modern microbiology.
How to Identify Parts of a Compound Microscope
Trying to learn the parts of a compound light microscope? Here are clear descriptions and detailed photos to help you become a master of the microscope.
Tips for Using a Compound Light Microscope
Students new to using microscopes typically get frustrated when first trying to look at specimens. Here's how to overcome some of the most common problems.
Probiotic Bacteria that Promote Digestive Health
Probiotics are beneficial bacteria naturally found in the digestive tract and vagina and often used to supplement dairy foods such as yogurt.
Norovirus Infection - The Stomach Flu Bug
Norovirus infection is the latest plague in the news. Learn about the symptoms, transmission and prevention of this acute gastrointestinal virus.
Does Mouthwash Kill Bacteria? Data Analysis
After completing a classroom experiment in which students take oral samples to see if mouthwash reduces the number and variety of bacteria, this is how data are examined.
Does Mouthwash Kill Bacteria? Data Collection
In this simple classroom experiment students take oral swab samples to see if the use of mouthwash reduces the number and variety of oral bacteria found in the mouth.
How Do Antibiotics Work to Kill Bacteria?
Antibiotics are drugs used to fight bacterial infections. How do these medications work to kill bacteria without harming human cells?
Sputnik - Now a Virus Satellite
Newly discovered cell-sized viruses have an infecting satellite virus, with implications for viruses as life forms, cell origins, the cause of disease and its treatment.
Using Heat to Destroy and Kill Microbes
Heat, applied through boiling and autoclaving is used to sterilize. But how does heat destroy cells and viruses? Here is a simple explanation of heat's mode of action.
Focusing and Finding Specimens with a Microscope
Students new to using a microscope often get frustrated when trying to view objects, particularly at higher magnifications. Here's how to do it sans the aggravation.
What Is a Compound Light Microscope?
Learn about the features of a compound light microscope, the importance of contrast and how to determine total magnification of ocular and objective lenses.
Making Sense of the Genetics of RNA Viruses
The genetic material of a virus can be either DNA or RNA, and if it is single-stranded RNA, the biologist must then make sense of the type of strand.
Viral DNA and RNA Genomes
Viral genomes might be small, but, as dsDNA, ssDNA, dsRNA or SS RNA, they show much more variation in form than is found in the genetic material of living cells.
How to Prepare a Microscope Slide of Bacteria
In order to view individual bacteria through a light microscope, a bacterial smear must be attached to a slide and then stained. Here is the procedure.
Viewing Bacteria under Oil Immersion
The oil immersion objective lens must be used in order to see individual bacteria through a light microscope. Here are the steps required to get a sample in focus.
Differential Staining & Bacterial Controls
Bacterial controls are often used with differential stains as examples of typical positive and negative stain reactions; helpful references when identifying unknowns.
Blood Agar (BAP) Bacterial Growth Medium
Blood Agar is a bacterial growth medium that can distinguish normal from pathogenic bacteria based on the effect of bacterial hemolytic enzymes on red blood cells.
MacConkey's Agar (MAC) Bacterial Growth Medium
MacConkeys Agar is a special bacterial growth medium that is selective for Gram- bacteria and can differentiate those bacteria that are able to ferment lactose.
Tests for Identification of Bacteria
Gram, Acid Fast and Endospore stains; MacConkey's, Mannitol Salt, and Blood Agar media as well as the API-20 test strip all provide information to identify microbes.
Selective Bacterial Growth Media
Selective media inhibit the growth of certain microbes, providing general information regarding the bacteria that are able to grow on these specialized types of agar.
Glycocalyx Bacterial Surface Coating
Some bacteria have an additional layer outside of the cell wall called the glycocalyx. This coating of macromolecules protects the cell and helps it adhere to surfaces.